Alberti S, Gladfelter A, Mittag T. Considerations and challenges in studying liquid-liquid phase separation and biomolecular condensates. Cell. 2019;176:419–34.
Google Scholar
Ozsvar J, Yang C, Cain SA, Baldock CL, Tarakanova A, Weiss AS. Tropoelastin and elastin assembly. Front Bioeng Biotech. 2021;9:643110.
Karamanos NK, Theocharis AD, Piperigkou Z, Manou D, Passi A, Skandalis SS, et al. A guide to the composition and functions of the extracellular matrix. FEBS J. 2021;288:6850–912.
Google Scholar
Schmelzer CEH, Duca L. Elastic fibers: formation, function, and fate during aging and disease. FEBS J. 2022;289:3704–30.
Google Scholar
Tamburro AM, Bochicchio B, Pepe A. Dissection of human tropoelastin: Exon-by-exon chemical synthesis and related conformational studies. Biochemistry. 2003;42:13347–62.
Google Scholar
Vrhovski B, Weiss AS. Biochemistry of tropoelastin. Eur J Biochem. 1998;258:1–18.
Google Scholar
Rauscher S, Baud S, Miao M, Keeley FW, Pomès R. Proline and glycine control protein self-organization into elastomeric or amyloid fibrils. Structure. 2006;14:1667–76.
Google Scholar
Muiznieks LD, Keeley FW. Proline periodicity modulates the self-assembly properties of elastin-like polypeptides. J Biol Chem. 2010;285:39779–89.
Google Scholar
Urry DW. Molecular machines: How motion and other functions of living organisms can result from reversible chemical changes. Angew Chem Int Ed. 1993;32:819–41.
Le DHT, Sugawara-Narutaki A. Elastin-like polypeptides as building motifs toward designing functional nanobiomaterials. Mol Syst Des Eng. 2019;4:545–65.
Google Scholar
McDaniel JR, Radford DC, Chilkoti A. A unified model for de novo design of elastin-like polypeptides with tunable inverse transition temperatures. Biomacromolecules. 2013;14:2866–72.
Google Scholar
Quiroz F, Chilkoti A. Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers. Nature Mater. 2015;14:1164–71.
Google Scholar
Chow D, Nunalee ML, Lim DW, Simnick AJ, Chilkoti A. Peptide-based biopolymers in biomedicine and biotechnology. Mater Sci Eng R Rep. 2008;62:125–55.
Rodríguez-Cabello JC, Arias FJ, Rodrigo MA, Girotti A. Elastin-like polypeptides in drug delivery. Adv Drug Delive Rev. 2016;97:85–100.
Jenkins IC, Milligan JJ, Chilkoti A. Genetically encoded elastin-like polypeptides for drug delivery. Adv Healthc Mater. 2021;10:2100209.
Google Scholar
Díez Pérez T, Tafoya AN, Peabody DS, Lakin MR, Hurwitz I, Carroll NJ, et al. Isolation of nucleic acids using liquid–liquid phase separation of pH-sensitive elastin-like polypeptides. Sci Rep. 2024;14:10157.
Google Scholar
Chen C, Ganar KA, de Haas RJ, Jarnot N, Hogeveen E, de Vries R, et al. Elastin-like polypeptide coacervates as reversibly triggerable compartments for synthetic cells. Commun Chem 2024;7:198.
Google Scholar
Ge X, Conley AJ, Brandle JE, Truant R, Filipe CDM. In vivo formation of protein based aqueous microcompartments. J Am Chem Soc. 2009;131:9094–9.
Google Scholar
Pastuszka MK, Janib SM, Weitzhandler I, Okamoto CT, Hamm-Alvarez SA, MacKay JA. A Tunable and reversible platform for the intracellular formation of genetically engineered protein microdomains. Biomacromolecules. 2012;13:3439–44.
Google Scholar
Li Z, Tyrpak DR, Lien CL, MacKay JA. Tunable assembly of protein-microdomains in living vertebrate embryos. Adv Biosyst. 2018;2:1800112.
Google Scholar
Vidal Ceballos A, Díaz AJA, Preston JM, Vairamon C, Shen C, Koder RL, et al. Liquid to solid transition of elastin condensates. Proc Natl Acad Sci USA. 2022;119:e2202240119.
Google Scholar
Le DHT, Hanamura R, Pham DH, Kato M, Tirrell DA, Okubo T, Sugawara-Narutaki A. Self-assembly of elastin–mimetic double hydrophobic polypeptides. Biomacromolecules. 2013;14:1028–1034.
Google Scholar
Le DHT, Okubo T, Sugawara-Narutaki A. Beaded nanofibers assembled from double-hydrophobic elastin-like block polypeptides: Effects of trifluoroethanol. Biopolymers. 2015;103:175–85.
Google Scholar
Le DHT, Kawakami R, Teraoka Y, Okubo T, Sugawara-Narutaki A. Crosslinking-assisted stabilization of beaded nanofibers from elastin-like double hydrophobic polypeptides. Chem Lett. 2015;44:530–2.
Google Scholar
Le DHT, Tsutsui Y, Sugawara-Narutaki A, Yukawa H, Baba Y, Ohtsuki C. Double-hydrophobic elastin-like polypeptides with added functional motifs: Self-assembly and cytocompatibility. J Biomed Mater Res A. 2017;105:2475–84.
Google Scholar
Natsume K, Nakamura J, Sato K, Ohtsuki C, Sugawara-Narutaki A. Biological properties of self-assembled nanofibers of elastin-like block polypeptides for tissue-engineered vascular grafts: Platelet inhibition, endothelial cell activation and smooth muscle cell maintenance. Regen Biomater. 2023;10:rbac111.
Google Scholar
Sugawara-Narutaki A, Yasunaga S, Sugioka Y, Le DHT, Kitamura I, Nakamura J, et al. Rheology of dispersions of high-aspect-ratio nanofibers assembled from elastin-like double-hydrophobic polypeptides. Int J Mol Sci. 2019;20:6262.
Google Scholar
Sugioka Y, Nakamura J, Ohtsuki C, Sugawara-Narutaki A. Thixotropic hydrogels composed of self-assembled nanofibers of double-hydrophobic elastin-like block polypeptides. Int J Mol Sci. 2021;22:4104.
Google Scholar
Bressan GM, Pasquali-Ronchetti I, Fornieri C, Mattioli F, Castellani I, Volpin D. Relevance of aggregation properties of tropoelastin to the assembly and structure of elastic fibers. J Ultrastruct Mol Struct Res. 1986;94:209–16.
Google Scholar
Roccatano D, Colombo G, Fioroni M, Mark AE. Mechanism by which 2,2,2-trifluoroethanol/water mixtures stabilize secondary-structure formation in peptides: A molecular dynamics study. Proc Natl Acad Sci USA. 2002;99:12179–84.
Google Scholar
Gupta P, Mandal BB. Tissue-engineered vascular grafts: emerging trends and technologies. Adv Funct Mater. 2021;31:2100027.
Google Scholar
Leal BBJ, Wakabayashi N, Oyama K, Kamiya H, Braghirolli DI, Pranke P. Vascular tissue engineering: polymers and methodologies for small caliber vascular grafts. Front Cardiovasc Med. 2020;7:592361.
Google Scholar
Barnes MJ, MacIntyre DE. Platelet-reactivity of isolated constituents of the blood vessel wall. Hemostasis. 1979;8:158–70.
Waterhouse A, Wise SG, Ng MKC, Weiss AS. Elastin as a nonthrombogenic biomaterial. Tissue Eng Part B Rev. 2011;17:93–99.
Nguyen TU, Bashur CA, Kishore V. Impact of elastin incorporation into electrochemically aligned collagen fibers on mechanical properties and smooth muscle cell phenotype. Biomed Mater. 2016;11:025008.
Google Scholar
Sugiura T, Agarwal R, Tara S, Yi T, Lee YU, Breuer CK, et al. Tropoelastin inhibits intimal hyperplasia of mouse bioresorbable arterial vascular grafts. Acta Biomater. 2017;52:74–80.
Google Scholar
Wagenseil JE, Mecham RP. Vascular extracellular matrix and arterial mechanics. Physiol Rev. 2009;89:957–89.
Google Scholar
Daamen WF, Nillesen STM, Hafmans T, Veerkamp JH, van Luyn MJA, van Kuppevelt TH. Tissue response of defined collagen–elastin scaffolds in young and adult rats with special attention to calcification. Biomaterials. 2005;26:81–92.
Google Scholar
Sugawara-Narutaki A, Nakamura J, Ohtsuki C. Elastin-like hydrogels as tissue regeneration scaffolds. In: Oliveira JM, Silva-Correia J, Reis RL, editors. Hydrogels for tissue engineering and regenerative medicine. From fundamentals to applications. Cambridge, Massachusetts: Academic Press; 2023. p. 65–77.
Mithieux SM, Rasko JEJ, Weiss AS. Synthetic elastin hydrogels derived from massive elastic assemblies of self-organized human protein monomers. Biomaterials. 2004;25:4921–7.
Google Scholar
Annabi N, Mithieux SM, Zorlutuna P, Camci-Unal G, Weiss AS, Khademhosseini A. Engineered cell-laden human protein-based elastomer. Biomaterials. 2013;34:5496–505.
Google Scholar
Annabi N, Zhang YN, Assmann A, Sani ES, Cheng G, Lassaletta AD, et al. Engineering a highly elastic human protein-based sealant for surgical applications. Sci Transl Med. 2017;9:eaai7466.
Google Scholar
Annabi N, Tsang K, Mithieux SM, Nikkhah M, Ameri A, Khademhosseini A, et al. Highly elastic micropatterned hydrogel for engineering functional cardiac tissue. Adv Funct Mater. 2013;23:4950–9.
Google Scholar
Betre H, Setton LA, Meyer DE, Chilkoti A. Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair. Biomacromolecules. 2002;3:910–6.
Google Scholar
Misbah MH, Quintanilla L, Alonso M, Rodríguez-Cabello JC. Evolution of amphiphilic elastin-like co-recombinamer morphologies from micelles to a lyotropic hydrogel. Polymer. 2015;81:37–44.
Google Scholar
Nagapudi K, Brinkman WT, Thomas BS, Park JO, Srinivasarao M, Wright E, et al. Viscoelastic and mechanical behavior of recombinant protein elastomers. Biomaterials. 2005;26:4695–706.
Google Scholar
Jordan SW, Haller CA, Sallach RE, Apkarian RP, Hanson SR, Chaikof EL. The effect of a recombinant elastin-mimetic coating of an ePTFE prosthesis on acute thrombogenicity in a baboon arteriovenous shunt. Biomaterials. 2007;28:1191–7.
Google Scholar
Sallach RE, Cui W, Balderrama F, Martinez AW, Wen J, Haller CA, et al. Long-term biostability of self-assembling protein polymers in the absence of covalent crosslinking. Biomaterials. 2010;31:779–91.
Google Scholar
Glassman MJ, Olsen BD. Arrested phase separation of elastin-like polypeptide solutions yields stiff, thermoresponsive gels. Biomacromolecules. 2015;16:3762–73.
Google Scholar
Reguera J, Lagarón JM, Alonso M, Reboto V, Calvo B, Rodríguez-Cabello JC. Thermal behavior and kinetic analysis of the chain unfolding and refolding and of the concomitant nonpolar solvation and desolvation of two elastin-like polymers. Biomacromolecules. 2003;36:8470–6.
Google Scholar
Glassman MJ, Avery RK, Khademhosseini A, Olsen BD. Toughening of thermoresponsive arrested networks of elastin-like polypeptides to engineer cytocompatible tissue scaffolds. Biomacromolecules. 2016;17:415–26.
Google Scholar